| 2019-10-12 14:30:46October 12, 2019 | Hartpury University RFC vs Doncaster Knights | 14:30:462:30 pm | Hartpury College |
| 2019-10-18 20:00:05October 18, 2019 | Newcastle Falcons vs Hartpury University RFC | 20:00:058:00 pm | Newcastle Falcons |
| 2019-10-25 19:45:17October 25, 2019 | Hartpury University RFC vs Nottingham | 19:45:177:45 pm | Hartpury College |
| 2019-11-02 14:15:07November 2, 2019 | Ampthill vs Hartpury University RFC | 14:15:072:15 pm | Ampthill & District Community Rufc |
| 2019-11-08 19:45:20November 8, 2019 | Hartpury University RFC vs London Scottish | 19:45:207:45 pm | Hartpury College |
| 2019-11-16 14:30:27November 16, 2019 | Hartpury University RFC vs Yorkshire Carnegie | 14:30:272:30 pm | Hartpury College |
| 2019-12-22 14:30:27December 22, 2019 | Cornish Pirates vs Hartpury University RFC | 14:30:272:30 pm | Cornish Pirates |
| 2020-01-11 13:30:13January 11, 2020 | Hartpury University RFC vs Jersey Reds | 13:30:131:30 pm | Hartpury College |
| 2020-01-18 15:00:55January 18, 2020 | Bedford Blues vs Hartpury University RFC | 15:00:553:00 pm | Bedford Blues |
| 2020-01-25 14:30:55January 25, 2020 | Hartpury University RFC vs Coventry | 14:30:552:30 pm | Hartpury College |
| 2020-02-01 15:00:03February 1, 2020 | Ealing Trailfinders vs Hartpury University RFC | 15:00:033:00 pm | Ealing Trailfinders |
| 2020-02-08 14:30:09February 8, 2020 | Hartpury University RFC vs Newcastle Falcons | 14:30:092:30 pm | Hartpury College |
| 2020-02-14 19:45:31February 14, 2020 | Nottingham vs Hartpury University RFC | 19:45:317:45 pm | Nottingham Rugby |
| 2020-02-28 19:45:10February 28, 2020 | Hartpury University RFC vs Ampthill | 19:45:107:45 pm | Hartpury College |
| 2020-03-14 15:00:39March 14, 2020 | London Scottish vs Hartpury University RFC | 15:00:393:00 pm | London Scottish |
| 2020-03-22 15:00:27March 22, 2020 | Yorkshire Carnegie vs Hartpury University RFC | 15:00:273:00 pm | Yorkshire Carnegie |
| 2020-03-27 19:45:43March 27, 2020 | Hartpury University RFC vs Cornish Pirates | 19:45:437:45 pm | Hartpury College |
| 2020-04-04 15:00:16April 4, 2020 | Jersey Reds vs Hartpury University RFC | 15:00:163:00 pm | Jersey Reds |
| 2020-04-11 14:30:41April 11, 2020 | Hartpury University RFC vs Bedford Blues | 14:30:412:30 pm | Hartpury College |
| 2020-04-25 15:00:05April 25, 2020 | Coventry vs Hartpury University RFC | 15:00:053:00 pm | Coventry Rugby Club |
| 2020-05-02 14:30:21May 2, 2020 | Hartpury University RFC vs Ealing Trailfinders | 14:30:212:30 pm | Hartpury College |
| 2020-05-09 14:30:19May 9, 2020 | Doncaster Knights vs Hartpury University RFC | 14:30:192:30 pm | Doncaster Knights |
